Increasing POMC Positive Neurons and Ameliorating the Brain Inflammation Played an Important Role in DJB Improving Glucose Homeostasis in Diabetic Rats

This study was focused on the effect of duodenal-jejunal bypass (DJB) on brain inflammation, activation brain insulin signaling and POMC positive neurons in type 2 diabetic (T2DM) rats. T2DM rats were induced by high-fat diet (HFD) and low dose streptozotocin (STZ) injection. Twelve T2DM rats were randomly divided into 2 groups, DJB group (T2DM-DJB, n=6) and T2DM group (T2DM-Sham, n=6). Six health Wistar rats were used as normal control rats (Wistar-Control, n=6). The results showed DJB group was significantly reduced the HOMA-IR and increased HOMA-ISI of T2DM rats 6 weeks after surgery. The Q-RT-PCR and Western-blot results showed DJB intervene significantly decreased the expression of inflammatory factors IL-6, TNF-α, IL-1β and NF-κB in the brain. Our data also showed that DJB activated the brain insulin by increasing the expression of insulin, IRS1 and IRS2 in the brain of T2DM rats. Immunohistochemical assay showed that DJB increased the number of POMC positive neurons in brain, and increased the expression of BDNF in neurons. Those results indicated that DJB might fulfill the antidiabetic effect by increasing POMC positive neurons and decreasing the brain inflammation in T2DM rats. Disclosure H. Wang: None. B. Jiang: None. J. Li: None. W. Wang: None. N. Li: None. Q. Yan: None. Z. Gao: None. M. Qu: None. Funding National Natural Science Foundation of China (81871892, 31671208, 81500798, 81471048); Shandong Province Natural Science Foundation (ZR2015HL128); Technology Development Plan of Weifang (2018YX027)