Cultivation technology development of Rhodothermus marinus DSM 16675

This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LB malt ) resulted in higher cell density (OD 620 = 6.6) than use of MB malt (OD 620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD 620 = 20) in LB malt and eightfold (OD 620 = 14) in MB malt . In both single and sequential batches, the production of carotenoids and EPSs using LB malt was detected in the exponential phase and stationary phase, respectively, while in MB malt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity ( Q E ) of 0.67 (mg/L h) in MB malt and the polymer contained xylose. In LB, Q E was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.