Fuel Use During Exercise At Altitude In Women With Glucose-Fructose Ingestion

Purpose This study compared the coingestion of glucose and fructose on exogenous and endogenous substrate oxidation during prolonged exercise at terrestrial high altitude (HA) versus sea level, in women. Method Five women completed two bouts of cycling at the same relative workload (55% W-max) for 120 min on acute exposure to HA (3375 m) and at sea level (similar to 113 m). In each trial, participants ingested 1.2 g center dot min(-1) of glucose (enriched with C-13 glucose) and 0.6 g center dot min(-1) of fructose (enriched with C-13 fructose) before and every 15 min during exercise. Indirect calorimetry and isotope ratio mass spectrometry were used to calculate fat oxidation, total and exogenous carbohydrate oxidation, plasma glucose oxidation, and endogenous glucose oxidation derived from liver and muscle glycogen. Results The rates and absolute contribution of exogenous carbohydrate oxidation was significantly lower at HA compared with sea level (effect size [ES] > 0.99, P < 0.024), with the relative exogenous carbohydrate contribution approaching significance (32.6% +/- 6.1% vs 36.0% +/- 6.1%, ES = 0.56, P = 0.059) during the second hour of exercise. In comparison, no significant differences were observed between HA and sea level for the relative and absolute contributions of liver glucose (3.2% +/- 1.2% vs 3.1% +/- 0.8%, ES = 0.09, P = 0.635 and 5.1 +/- 1.8 vs 5.4 +/- 1.7 g, ES = 0.19, P = 0.217), and muscle glycogen (14.4% +/- 12.2% vs 15.8% +/- 9.3%, ES = 0.11, P = 0.934 and 23.1 +/- 19.0 vs 28.7 +/- 17.8 g, ES = 0.30, P = 0.367). Furthermore, there was no significant difference in total fat oxidation between HA and sea level (66.3 +/- 21.4 vs 59.6 +/- 7.7 g, ES = 0.32, P = 0.557). Conclusions In women, acute exposure to HA reduces the reliance on exogenous carbohydrate oxidation during cycling at the same relative exercise intensity.