Determination of Aantibacterial Effect of the Epithelial and Mesenchymal Surfaces of Amniotic Membrane on Escherichia coli, Staphylococcus aureus

Background and Objectives: Human amniotic membrane due to its unique characteristics, has many applications various fields of medicine and tissue engineering. One of these characteristics is antibacterial property, which can be important in protection against infections after burn and surgery. This study was designed aiming at investigating the antibacterial effect of amnion on the growth of different bacterial strains and comparing this effect in epithelial and mesenchymal sides of the amnion. Methods: In this study, the antibacterial effect of amniotic membrane was evaluated on three standard bacterial strains Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and two clinical isolated strain of E. coli ( E. coli T3 and E. coli T4), and their susceptibility was measured. A modified disk diffusion test was used to assess the antibacterial effect. In this method, amnion was placed on the cultured agar surface as an antibiotic releasing disk. Statistical analysis was performed using analysis of variance and Tukey's tests. Results: The vicinity of amnion membrane caused inhibition zone against Pseudomonas aeruginosa ATCC 27 853 and two clinical isolated strains of E. coli . Formation of Inhibition zone in Pseudomonas aeruginosa ATCC 27853 and two clinical isolated strains of E. coli had no relationship with the epithelial or mesenchymal side of amniotic membrane. Conclusion: According to the results of this study, it seems that the type of bacterial strain has a role in the antibacterial effect of amnion membrane. In addition, both sides of amnion (epithelial and mesenchymal) may have similar antibacterial effect.