Application of a Micro-Cold Atmospheric Plasma Device (μCAP) in Vitro and Vivo for Brain Cancer Therapy

Cold atmospheric plasma (CAP) has received great attention due to their remarkable abilities in plasma medicine. Many scientists have successfully elucidated the potential of CAP as a novel technology against cancer1. CAP may be generated by a range of different plasma devices such as plasma jet, dielectric barrier discharge, corona discharge, gliding arc, etc2. However, most cancers occur inside the body and plasma direct irradiation is impractical as a clinical cancer therapy due to its high voltage, the formation of discharge in the organ, gas delivery, and plasma probe volume3,4. In addition, the heat inside the organ should be controllable and normal tissue should be protected from damage. In recent years, tiny, flexible, and easy to handle CAP devices have been considered for use in medical applications. In this context, here we developed a Micro-CAP (uCAP) device employing helium gas. The main objective was to investigate the effect of uCAP on glioblastoma cancer cells (U87) and glioblastoma tumors in the murine brain.