Repeated Prolonged Exercise Decreases Maximal Fat Oxidation in Older Men

Introduction/Purpose: Fat metabolism and muscle adaptation was investigated in six older trained men (age, 61 +/- 4 yr; (V) over dotO(2max), 48 +/- 2 mL.kg(-1).min(-1)) after repeated prolonged exercise). Methods: A distance of 2706 km (1681 miles) cycling was performed over 14 d, and a blood sample and a muscle biopsy were obtained at rest after an overnight fast before and 30 h after the completion of the cycling. (V) over dotO(2max) and maximal fat oxidation were measured using incremental exercise tests. HR was continuously sampled during cycling to estimate exercise intensity. Results: The daily duration of exercise was 10 h and 31 +/- 37 min, and the mean intensity was 53% +/- 1% of (V) over dotO(2max). Body weight remained unchanged. (V) over dotO(2max) and maximal fat oxidation rate decreased by 6% +/- 2% (P = 0.04) and 32% +/- 8% (P < 0.01), respectively. The exercise intensity that elicits maximal fat oxidation was not significantly decreased. Plasma free fatty acid (FA) concentration decreased (P < 0.002) from 500 +/- 77 mu mol.L-1 to 160 +/- 38 mu mol.L-1. Plasma glucose concentration as well as muscle glycogen, myoglobin, and triacylglycerol content remained unchanged. Muscle citrate synthase and beta-hydroxy-acyl-CoA-dehydrogenase activities were unchanged, but the protein expression of HKII, GLUT4, and adipose triacylglycerol lipase were significantly increased. Conclusions: Overall, the decreased maximal fat oxidation was probably due to lower exogenous plasma fatty acid availability and the muscle adaptation pattern indicates an increased glucose transport capacity and an increased muscle lipolysis capacity supporting an increased contribution of exogenous glucose and endogenous fat during exercise.