microRNA-27a regulates maturation process and cytokine in LPS-stimulated dendritic cells

Objective: To explore the effects of miR-27 a on the phenotype and cytokine secretion in LPS-stimulated dendritic cells. Methods: Murine bone marrow-derived dendritic cells were transfected with miR-27 a mimics and negative control mimics,and then stimulated by LPS for 24 hours. Dendritic cells exposed to LPS were collected for analysis of the DC immunophenotype by flow cytometry and supernatants were collected to determine cytokine lever. Moreover,the capability of stimulating allogeneic CD4+T cell proliferation was measured by MLR( mixed lymphocyte reaction). Results: The levels of MHCⅡ,CD80,and CD86 were significantly increased in LPS-stimulated dendritic cells when compared with im DC( P 0. 001). Transfection with miR-27 a mimics resulted in significantly lower expression levels in levels of MHCⅡ,CD80,and CD86( P 0. 001). For cytokine secretion,transfection with miR-27 a mimics enhanced IL-10 production( P 0. 01) and reduced the production of IL-12( P 0. 05). For MLR,transfection with miR-27 a mimics suppressed allogeneic CD4+T cell proliferation. Conclusion: MiR-27 a may play critical roles in regulating the maturation process and cytokine secretion in LPS-stimulated dendritic cells.