The detective value of high-risk human papillomavirus genotype 16 and 18 DNA in screening cervical lesions

Objective To evaluate the detective value of high-risk human papillomavirus(HPV) genotype 16 and 18(HPV16,18) in screening cervical lesions.Methods By histological biopsy,209 cases were cancer and percancerous lesions [cervical intraepithelial neoplasia(CIN) Ⅰ,CIN Ⅱ,CIN Ⅲ and invasive cervical cancer],and 296 cases were inflammation,polyp and other benign lesions.HPV16,18 DNA and liquid-based cytology were used to detect the cases with cancer and percancerous lesions by histological biopsy.Comparing the results of the 2 methods,HPV16,18 infectious rates,sensitivity,specificity and negative predictive value were statistically analyzed.Results Of 209 patients with cancer and precancerous lesions,158 cases were positive of HPV16,18,and 128 cases were positive of liquid-based cytology [low-grade squamous intraepithelial lesion(LSIL),atypical squamous cells,cannot exclude a high-grade squamous intraepithelial lesion(ASC-H),high-grade squamous intraepithelial lesion(HSIL) and squamous cell carcinoma].Among the 296 patients with benign lesions,43 cases were positive of HPV16,18,and 2 cases were positive of liquid-based cytology.The results of cancer and precancerous lesions showed that the positive rate of HPV16,18 DNA was higher than that of liquid-based cytology(χ2=12.69,P0.01).The sensitivity,specificity and negative predictive value of HPV16,18 DNA were 75.6%,85.5% and 83.2% respectively,and 61.2%,99.3% and 78.4% of liquid-based cytology.The HPV16,18 positive rate of CIN Ⅱ was obviously higher than that of CINⅠ(χ2=6.69,P0.05).Conclusions The occurrence of cervical cancer and percancerous lesions is closely related to HPV16,18 infection.Compared with liquid-based cytology,HPV16,18 DNA in cervical cancer and percancerous lesions has a higher sensitivity,lower specificity and higher negative predictive value.HPV16,18 DNA has an important clinical value for screening cervical benign lesions,and it is better than liquid-based cytology.