Assessment of genetic diversity in 29 rose germplasms using SCoT marker

Roses are well known for commercial floriculture and greatly used in the field of perfumery, soap, cosmetics, jams & jelly and essential oil production. Due to interspecific hybridization, a large number of hybrids and cultivated varieties of rose are recognized which reveals distinguishable features in flowers such as size, shape, and color. Apart from this, the geographical distribution and polyploidy also make Rose genus more complex. Therefore, the present study was undertaken for the identification and characterization of genetic variation within 29 rose accessions through Start codon targeted polymorphism (SCoT) markers. Out of 36 primers, 32 revealed polymorphic amplification profile in 29 rose accessions with amplification ranging from 150 bp to 1.2 kb. A total of 299 polymorphic amplicons were obtained, ranging from 4 to 19 amplicons with an average of 9.34 amplicons per primer. The polymorphic information content (PIC) ranged from 0.42 to 0.92 with an average of 0.78. The dendrogram was constructed to establish genetic relationship among 29 different accessions using Neighbor-joining and Nei-Li matching coefficient. The distinguishable genetic background and a high degree of variation in the rose genotypes successfully exhibited by the SCoT markers may serve as a valuable aid in Rose improvement strategy.