Sleep Deprivation and Late Bedtime Impair Sperm Health Through Increasing Antisperm Antibody Production: A Prospective Study of 981 Healthy Men

Background: The aim of this study was to investigate the effects of sleep duration and bedtime on sperm health, and the possible mechanism involved. Material/Methods: We randomly divided 981 healthy Chinese men into groups according to research-set bedtimes (A= 8-10 PM, B= after 10 PM, and C=after midnight) and sleep durations: group 1=< 6.0 h (short), group 2=7.0-8.0 h (average), and group 3=> 9.0 h (long). Sperm morphology, count, survival, and motility were examined according to sleep patterns. Antisperm antibody (ASA) production in semen was determined. Results: Sperm counts and their survival rates were lower in the short sleepers as compared to others within each group (all P < 0.01). The lower counts and survival rates were observed in different bedtimes, with significant differences found between measurements of C1 vs. A1 and C2 vs. A2 or B2 (all P < 0.05 or 0.01). Semen motility was lower in the short sleepers as compared to the average and long sleepers (all P < 0.01). There were differences in the bedtime-related results between measurements of C1 vs. A1 or B1 (P < 0.05 or 0.01). Additionally, the population proportion for the ASA-positive participates and incidence of the ASA-expressed population obviously increased in the short sleepers as compared to others within each group (all P < 0.05). Conclusions: Short and long sleep durations and late bedtime were associated with impaired sperm health in the study cohort, partly through increasing ASA production in the semen.