The Role Of Inflammation And Free Radicals In Ad Type Of Dementia

Background: The pathogenic action of toxic free radicals is one of the hypotheses put forward to account for the onset of Alzheimer's (AD) dementia. Purpose: Study aimed at investigation of blood several free toxic radicals relation with cognitive decline in probable AD patients. Methods: The total of 75 probable AD patients had been investigated. Diagnosis was established according to NINCDS-ADRDA criteria. Brain visualized by conventional MRI. Neurological and neuropsychological examination had been performed. Cognitive status was researched 2 times with 1 year period between examinations by MMSE. Control comprised 30 age matched healthy persons. Blood free radicals: Hydroxyl radical (OH-) and lipoperoxyradical (LOO-) were researched 2 times in accordance with cognitive examination by Electron Paramagnetic Resonance (EPR) method. The α-phenil-tert-butilitron (PBN) (SIGMA) was used as LOO- trap. EPR signal intensity was measured in millimeters on milliliter blood matter. Statistics performed by SPSS-11.0. Results: During both examinations EPR signals of blood free radicals OH- and LOO- found to be significantly higher in AD patients as compared to control (p < 0.001), while the second examination of AD patients showed the increased EPR signals of LOO- against the first data(p < 0.05) but the EPR specters of OH- were non-significantly increased (p > 0.05). Positive correlation revealed between the blood LOO- and the cognitive decline in selected AD patients(r = +0.47; p < 0.05). Conclusion: Membrane lipid degradation product lipoperoxyradical (LOO-) seem to be involved in cognitive decline in Alzheimer’s type of dementia.