Identification Of A New Mechanism Of Microrna Turnover From Mir-106a-363 Cluster Leading To Epithelial-To-Mesenchymal Transition In Prostate Cancer

Background: Prostate cancer (PCa) is the second leading cause of cancer mortality in US. The majority of PCa mortality is due to the recurrent of metastatic castration resistant PCa. The acquisition of epithelial-to-mesenchymal transition (EMT) signifies PCa metastasis. In particular, altered microRNAs (miRNAs) expression is closely associated with PCa progression from prostatic intraepithelial neoplasia to metastatic adenocarcinoma. miRNAs are small noncoding RNAs regulating approximately 60% protein-coding genes by post-transcriptional suppression or translational inhibition. miRNA gene expression at post-transcriptional level becomes more complicated when multiple miRNAs derived from the same cluster generate a polycistronic primary transcript, and each individual miRNA displays different expression profile and functional role. miRNA-363 (miR-363) belongs to the miR-106a-363 cluster containing miR-106a, miR-18b, miR-20b, miR-19b-2, miR-92a-2 and miR-363. Unlike the other five miRNAs which are closely resemble to the oncogenic miR-17-92 cluster in their seed sequence and function, miR-363 has been implicated to play a tumor suppressor role in several types of cancer, indicating a different regulatory mechanism of miR-363 from the miR-106a-363 cluster. Interferon-induced tetratricopeptide repeat 5 (IFIT5) is first characterized as a viral RNA binding protein and has been shown to directly bind to cellular tRNA, which partially shared a structural similarity with precursor miRNAs (pre-miRNAs). However, until now, there is no report indicating the RNA recognition role of IFIT5 in miRNA biogenesis machinery. Results: In the present study, miR-363 was clearly identified as a tumor suppressor miRNA by inhibiting EMT in PCa cells via targeting slug/SNAI2. More importantly, we have demonstrated, for the first time, that IFIT5 is able to recognize a unique structure at the 5′ end of precursor miR-363, which facilitates pre-miR-363 degradation by the recruitment of a 5′-3′exoribonuclease, XRN1. Meanwhile, we have also shown that the significant elevation of IFIT5 is detected in several PCa cells undergone EMT leading to highly metastatic potential. In addition, an inverse correlation between miR-363 and IFIT5 mRNA level was found in human PCa specimens. Conclusion: We unveil IFIT5 complex as a new post-transcriptional regulatory mechanism specific for miR-363 turnover at the precursor stage, which determines the stability and unique functional role of miR-363 distinct from other oncogenic miRNA members in the miR-106a-363 cluster. Overall, this study provides an insight of miRNA biogenesis machinery in cancer metastasis and new strategies of therapeutic intervention of PCa. Citation Format: U-Ging Lo, Rey-Chen Pong, Diane Yang, Jiancheng Zhou, Leah Gandee, Shu-Fen Tseng, Jer-Tsong Hsieh. Identification of a new mechanism of microRNA turnover from miR-106a-363 cluster leading to epithelial-to-mesenchymal transition in prostate cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2873. doi:10.1158/1538-7445.AM2015-2873