WAT apoC-I secretion: role in delayed chylomicron clearance in vivo and ex vivo in WAT in obese subjects
Journal of Lipid Research(2016)
摘要
Reduced white adipose tissue (WAT) LPL activity delays plasma clearance of TG-rich lipoproteins (TRLs). We reported the secretion of apoC-I, an LPL inhibitor, from WAT ex vivo in women. Therefore we hypothesized that WAT-secreted apoC-I associates with reduced WAT LPL activity and TRL clearance. WAT apoC-I secretion averaged 86.9 +/- 31.4 pmol/g/4 h and 74.1 +/- 36.6 pmol/g/4 h in 28 women and 11 men with BMI 27 kg/m(2), respectively, with no sex differences. Following the ingestion of a C-13-triolein-labeled high-fat meal, subjects with high WAT apoC-I secretion (above median) had delayed postprandial plasma clearance of dietary TRLs, assessed from plasma C-13-triolein-labeled TGs and apoB48. They also had reduced hydrolysis and storage of synthetic H-3-triolein-labeled (H-3)-TRLs in WAT ex vivo (i.e., in situ LPL activity). Adjusting for WAT in situ LPL activity eliminated group differences in chylomicron clearance; while adjusting for plasma apoC-I, H-3-NEFA uptake by WAT, or body composition did not. apoC-I inhibited in situ LPL activity in adipocytes in both a concentration- and time-dependent manner. There was no change in postprandial WAT apoC-I secretion. WAT apoC-I secretion may inhibit WAT LPL activity and promote delayed chylomicron clearance in overweight and obese subjects. We propose that reducing WAT apoC-I secretion ameliorates postprandial TRL clearance in humans.
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关键词
adipocytes,lipase/lipoprotein,triglycerides,obesity,lipolysis and fatty acid metabolism,fat storage,cardiometabolic risk,white adipose tissue,apolipoprotein C-I,triglyceride-rich lipoprotein
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