Microbial diversity of Bradysia odoriphaga and the rhizosphere microbes of chives

[Objectives] To understand microbial diversity in Bradysia odoriphaga and its environment. [Methods] Microorganisms in B. odoriphaga, and in the bulbs and roots of chives, and soil were separated, purified, and identified through ITS and 16 S r DNA genetic analysis. Physiological and biochemical analyses was then carried out for some representative bacteria. [Results] 25 microbial species were identified from the environment of B. odoriphaga. Beauveria bassiana and Cordyceps bassiana(Deuteromycotina), were found in both chive bulbs and soil. Most bacterial species belonged to the Proteobacteria and Firmicutes,the rest belonged to Actinobacteria and Bacteroidetes. 10, 15, 9, 6 bacterial species were identified in B. odoriphaga, and in the chive bulbs, roots and soil, respectively. [Conclusion] This study provides a theoretical foundation for investigating coevolution among chives, B. odoriphaga and microorganisms. We hope that it will also contribute to the symbiotic control of B. odoriphaga.