Effect of Ligustrazine on electroretinogram and expression of cAMP in vascular smooth muscle cell of retina arteriole after retina ischemia

OBJECTIVE To observe the effect of ligustrazine on retina ERG and Ops and expression of cAMP in vascular smooth muscle cell of retina arteriole after retina ischemia.METHODS Twenty rabbits were divided into two groups randomly: the ligustrazine group and the model group.Experimental retina ischemia-reperfusion injury was induced by ligatured optic nerve which was lasting for an hour on the right eye of all the rabbits.The ERG and Ops were recorded from ligustrazine group after 0h,24h,48h,72h following retina ischemia injury.The same testing was done on the model group.Put the rabbit to death,exteriorize the right eye and observe expression of cAMP in vascular smooth muscle cell of retina arteriole by immunofluorescence.RESULTS Ligustrazine could not improve the amplitude maximum retina ERG reaction on acute ocular ischemic animal model,however it could obviously promote the recovery of Ops of retina after 24h and 72h following retinal ischemia(P 0.05).Ligustrazine could improve expression of cAMP in vascular smooth muscle cell of retina after 72h ischemical injury.CONCLUSIONS The improved expression of cAMP in vascular smooth muscle cell of retina arteriole of Ligustrazine may be related to the Ops amplitude after retina ischemia.