Semi-Bionic Extraction of Effective Ingredient from Fishbone by High Intensity Pulsed Electric Fields

Semi-bionic extraction method and high intensity pulsed electric fields (PEF) were combined to rapidly extract effective ingredients (calcium, chondroitin sulfate and collagen) from fishbone. In order to simplify the analytical process, comprehensive index formula was determined by principal component analysis. Based on the principle of bionics, the optimal fluid pHs in human stomach, small intestine and large intestine are 2.0, 7.5 and 8.3. The simulated pHs were 2.0, 7.5 and 8.3, respectively, and variation of PEF parameters and the comprehensive evaluation index were determined by single factor experiments. Too low or too high liquid-material ratio and electric field strength were not conducive to the comprehensive evaluation index Y. The value of Y increased with the increase of pulse number. The maximum contents of calcium, chondroitin sulfate and collagen in the extract were obtained simultaneously by response surface methodology (RSM), that is, 19.8, 39.268 and 3.875 mg/mL, respectively The optimum condition is as the following: liquid-material ratio 11, electric field strength 22.79 kV/cm and pulse number 9. Practical ApplicationsSBE and PEF technique were combined to analyze the effective ingredients in fishbone. The prepared fishbone hydrolysate can be processed into multi-active and high-added-value compound products suitable for absorption and utilization by the digestive tract. This paper provides a new technique for comprehensive utilization of fishbone and fishbone waste, serves as a new clue for processing and extraction of natural products and provides a basis for promotion of SBE and PEF.