Shift in antibiotic resistance gene profiles associated with nanosilver during wastewater treatment.
FEMS MICROBIOLOGY ECOLOGY(2016)
摘要
This study investigated the response of antibiotic resistance genes (ARGs) to nanosilver (Ag) in lab-scale nitrifying sequencing batch reactors (SBRs), compared to Ag+-dosed and undosed controls. Quantitative polymerase chain reaction (q-PCR) targeting sul1, tet(O), ermB and the class I integron gene intI1 and corresponding RNA expression did not indicate measureable effects of nanoAg or Ag+ on abundance or expression of these genes. However, high-throughput sequencing based metagenomic analysis provided a much broader profile of gene responses and revealed a greater abundance of aminoglycoside resistance genes (mainly strA) in reactors dosed with nanoAg. In contrast, bacitracin and macrolide-lincosamide-streptogramin (MLS) resistance genes were more abundant in the SBRs dosed with Ag+. The distinct ARG profiles associated with nanoAg and Ag+ were correlated with the taxonomic composition of the microbial communities. This study indicates that nanoAg may interact with bacteria differently from Ag+ during biological wastewater treatment. Therefore, it cannot necessarily be assumed that nanosilver behaves identically as Ag+ when conducting a risk assessment for release into the environment.
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关键词
Antibiotic resistance genes,nanosilver,metagenomic analysis,biological wastewater treatment,sequencing batch reactors
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