Structure Elucidation, Conformation, And Configuration Of Cytotoxic 6-Heptyl-5,6-Dihydro-2h-Pyran-2-Ones From Hyptis Species And Their Molecular Docking To Alpha-Tubulin

Cytotoxic 6-heptyl-5,6-dihydro-2H-pyran-2-ones are chemical markers of Hyptis (Lamiaceae) and are responsible for some of the therapeutic properties of species with relevance to traditional medicine. The present investigation describes the isolation of known pectinolides A-C (1-3), in addition to the new pectinolides I-M (4-8), from two Mexican collections of H. pectinata by HPLC. The novel biosynthetically related monticolides A (9) and B (10) were also isolated by high-speed countercurrent chromatography from H. monticola, an endemic species of the Brazilian southeastern high-altitude regions. A combination of chemical correlations, chiroptical measurements, and Mosher ester NMR analysis was used to confirm their absolute configuration. The utility of DFT-NMR chemical shifts and J(H-H) calculations was assessed for epimer differentiation. Molecular docking studies indicated that 6-hepty1-5,6-dihydro-2H-pyran-2-ones have a high affinity for the pironetin-binding site of alpha-tubulin, which may be a possible mechanism contributing to the cytotoxic potential of these small and flexible molecules.