Corynebacterium defluvii sp. nov., isolated from Sewage

A Gram-positive, aerobic, non-motile, rod-shapeds, catalase-positive, and oxidase-negative strain, designated Y49 T , was isolated from sewage collected from Jilin Agricultural University, China. It grew at 20–40°C (optimum at 30°C), at pH 6.0–8.0 (optimum at 7.0) and at 0–1.0% sodium chloride (optimum at 0%). The major isoprenoid quinone was menaquinone-8 (MK-8) and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, four unidentified lipids, and two unidentified aminolipids. The peptidoglycan was meso -diaminopimelic acid. The cell-wall sugars were galactose, arabinose, and glucose. The fatty acids were C 9:0 , C 16:0 , C 16:1 ω 9 c , C 17:1 ω 9 c , C 18:3 ω 6 c (6,9,12), C 18:1 ω 9 c , and C 18:0 . The DNA G+C content was 51.4 mol%. Based on the 16S rRNA gene sequence analysis, the nearest phylogenetic neighbors of strain Y49 T were Corynebacterium efficiens DSM 44549 T (97.5%), Corynebacterium callunae DSM 20147 T (97.2%), Corynebacterium deserti GIMN 1.010 T (96.8%), Corynebacterium glutamicum ATCC 13032 T (96.4%), and other species belonging to this genus (92.3–95.4%). The DNA-DNA relatedness value between strain Y49 T and C. efficiens DSM 44549 T , C. callunae DSM 20147 T , C. deserti GIMN1.010 T , and C. glutamicum ATCC 13032 T was 25.5±2.0%, 21.1±1.0%, 16.5±0.5%, and 13.5±0.9%, respectively. Based on the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain Y49 T represents a novel species of the genus Corynebacterium , for which the name Corynebacterium defluvii sp nov. is proposed. The type strain is Y49 T (= KCTC 39731 T =CGMCC 1.15506 T ).