3D-Printed Silk Sericin-Based Hydrogel Scaffold：A Promising Visualized Dressing Material for Real-Time Monitoring of Wounds.

The wound dressing which can be convenient for real-time monitoring of wounds is particularly attractive and user-friendly. In this study, a nature-originated silk sericin (SS) based transparent hydrogel scaffold was prepared and evaluated for visualization wound care. The scaffold was fabricated from a hybrid interpenetrating-networks (IPN) hydrogel composed of SS and methacrylic anhydride modified gelatin (GelMA) by 3D printing. The scaffold transformed into high transparent hydrogel when swelling in PBS and thus made anything underneath easily read. The scaffold had high degree of swelling and presented regularly macroporous structure with pores around 400 μm×400 μm, which can help keeping the moist and apinoid environment for wound healing. Meanwhile, the scaffolds were conducive to adhesion and proliferation of L929 cells. Co-culture of HaCaT and HSF cells on the scaffold showed centralized proliferation of the two cells in distributed layers, respectively, denoting a promising comfortable environment for re-epithelialization. Moreover, in vivo studies demonstrated that the scaffold showed no excessive inflammatory reaction. In short, this work presented a SS based transparent hydrogel scaffold with steerable physical properties and excellent biocompatibility through 3D printing, pioneering the promising applications in visualization wound care and drug delivery.