Repair mechanism of mesenchymal stem cells derived from nasal mucosa in orbital fracture.

Objective: Mesenchymal stem cells (MSCs) derived from nasal mucosa are featured by high division and differentiation capacity, with large nuclei, obvious nucleoli and weak cytoplasmic basophily. Imaging examination, typically CT scan, is the gold standard for the diagnosis of orbital fracture. Methods: We isolated MSCs derived from goat nasal mucosa and built the calcification model so as to investigate the repair mechanism of nasal mucosa-derived MSCs in orbital fracture. Expressions of osteogenic markers Runx2, OCN, OPN and BSP were detected using western blot. Results: Nasal mucosa-derived MSCs were successfully isolated and passaged. Nestin was detected by immunofluorescence assay in the cells of the third generation. It was further confirmed that the isolated cells were nasal mucosa-derived MSCs. As indicated by alizarin red staining, the calcification model in nasal mucosaderived MSCs was successfully built. The relative expressions of Runx2 and OCN reached the highest level after osteogenic induction for 7 d, and the expressions of OPN and BSP were also high. But at 10 d, the expressions of all markers declined somewhat. At 14 d, the expressions of OPN and BSP reached the peak, but without significant differences compared with those at 7 d. Conclusion: The present study suggested that the repair effect of nasal mucosa-derived MSCs in orbital fracture is achieved by facilitating the expressions of osteogenic markers Runx2, OCN, OPN and BSP. However, the pathways of actions are unknown and further studies are required to elucidate the concrete mechanism.