Characterization of autonomous families of Tc1/mariner transposons in neoteleost genomes.

We report the comprehensive analysis of Tc1/mariner transposons in six species of neoteleost (cod, tetraodon, fugu, medaka, stickleback, and tilapia) for which draft sequences are available. In total, 33 Tc1/mariner families were identified in these neoteleost genomes, with 3–7 families in each species. Thirty of these are in full length and designed as autonomous families, and were classified into the DD34E (Tc1) and DD×D (pogo) groups. The DD34E (Tc1) group was further classified into five clusters (Passport-like, SB-like, Frog Prince-like, Minos-like, and Bari-like). Within the genomes of cod, tetraodon, fugu, and stickleback, the Tc1/mariner DNA transposons exhibit very low proliferation with <1% of genome. In contrast, medaka and tilapia display high accumulation of Tc1/mariner transposons with 2.91% and 5.09% of genome coverages, respectively. Divergence analysis revealed that most identified Tc1/mariner transposons have undergone one round of recent accumulation, followed by a decrease in activity. One family in stickleback (Tc1_6_Ga) exhibits a very recent and strong expansion, which suggests that this element is a very young invader and putatively active. The structural organization of these Tc1/mariner elements is also described. Generally, the Tc1/mariner transposons display a high diversity and varied abundance in the neoteleost genomes with current and recent activity.