Thymosin-Alpha 1 Expands Deficient Il-10-Producing Regulatory B Cell Subsets In Relapsing-Remitting Multiple Sclerosis Patients

Background: B cells are key pathogenic effectors in multiple sclerosis (MS) and several therapies have been designed to restrain B cell abnormalities by directly targeting this lymphocyte population.Objectives: Moving from our data showing a Toll-like receptor (TLR) 7-driven dysregulation of B cell response in relapsing-remitting multiple sclerosis (RRMS) and having found a low serum level of Thymosin-alpha 1 (T alpha 1) in patients, we investigated whether the addition of this molecule to peripheral blood mononuclear cells (PBMCs) would influence the expansion of regulatory B cell subsets, known to dampen autoimmune inflammation.Methods: Serum T alpha 1 level was measured by enzyme immunoassay. Cytokine expression was evaluated by Cytometric Bead Array (CBA), enzyme-linked immunosorbent assay (ELISA), and real-time reverse transcription polymerase chain reaction (RT-PCR). B cell subsets were analyzed by flow cytometry.Results: T alpha 1 pre-treatment induces an anti-inflammatory status in TLR7-stimulated RRMS PBMC cultures, reducing the secretion of pro-inflammatory interleukin (IL)-6, IL-8, and IL-1 beta while significantly increasing the regulatory IL-10 and IL-35. Indeed, T alpha 1 treatment enhanced expansion of CD19(+)CD24(+)CD38hi transitional-immature and CD24(low/neg)CD38(hi) plasmablast-like regulatory B cell subsets, which likely inhibit both interferon (IFN)-gamma and IL-17 production.Conclusion:: Our study reveals a deficient ability of B cells from MS patients to differentiate into regulatory subsets and unveils a novel anti-inflammatory and repurposing potential for T alpha 1 in MS targeting B cell response.