T-Tubule Remodelling Disturbs Localized Beta(2)-Adrenergic Signalling In Rat Ventricular Myocytes During The Progression Of Heart Failure

Aims Cardiomyocyte beta(2)-adrenergic receptor (beta(2)AR) cyclic adenosine monophosphate (cAMP) signalling is regulated by the receptors' subcellular location within transverse tubules (T-tubules), via interaction with structural and regulatory proteins, which form a signalosome. In chronic heart failure (HF), beta(2)ARs redistribute from T-tubules to the cell surface, which disrupts functional signalosomes and leads to diffuse cAMP signalling. However, the functional consequences of structural changes upon beta(2)AR-cAMP signalling during progression from hypertrophy to advanced HF are unknown.Methods and results Rat left ventricular myocytes were isolated at 4-, 8-, and 16-week post-myocardial infarction (MI), beta(2)ARs were stimulated either via whole-cell perfusion or locally through the nanopipette of the scanning ion conductance microscope. cAMP release was measured via a Forster Resonance Energy Transfer-based sensor Epac2-camps. Confocal imaging of di-8-ANNEPS-stained cells and immunoblotting were used to determine structural alterations. At 4-week post-MI, T-tubule regularity, density and junctophilin-2 (JPH2) expression were significantly decreased. The amplitude of local beta(2)AR-mediated cAMP in T-tubules was reduced and cAMP diffused throughout the cytosol instead of being locally confined. This was accompanied by partial caveolin-3 (Cav-3) dissociation from the membrane. At 8-week post-MI, the beta(2)AR-mediated cAMP response was observed at the T-tubules and the sarcolemma (crest). Finally, at 16-week post-MI, the whole cell beta(2)AR-mediated cAMP signal was depressed due to adenylate cyclase dysfunction, while overall Cav-3 levels were significantly increased and a substantial portion of Cav-3 dissociated into the cytosol. Overexpression of JPH2 in failing cells in vitro or AAV9. SERCA2a gene therapy in vivo did not improve beta(2)AR-mediated signal compartmentation or reduce cAMP diffusion.Conclusion Although changes in T-tubule structure and b2AR-mediated cAMP signalling are significant even at 4-week post-MI, progression to the HF phenotype is not linear. At 8-week post-MI the loss of beta(2)AR-mediated cAMP is temporarily reversed. Complete disorganization of beta(2)AR-mediated cAMP signalling due to changes in functional receptor localization and cellular structure occurs at 16-week post-MI.