Roseomonas chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from activated sludge

A Gram-negative, aerobic, short rod-shaped, pink-pigmented, non-motile bacterium, designated BUT-13 T , was isolated from activated sludge of an herbicide-manufacturing wastewater treatment facility in Jiangsu province, China. Growth was observed at 0–5.5 % NaCl, pH 6.0–9.0 and 12–37 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BUT-13 T is a member of the genus Roseomonas , and shows high sequence similarities to R. pecuniae N75 T (98.0 %) and R. rosea 173-96 T (97.5 %), and lower (<97 %) sequence similarities to all other Roseomonas species. Chemotaxonomic analysis revealed that strain BUT-13 T possesses Q-10 as the predominant ubiquinone; summed feature 8 (C 18:1 w 7 c and/or C 18:1 w 6 c ; 38.8 %), C 18:0 (16.6 %), C 16:0 (15.2 %), summed feature 3 (C 16:1 ω 6 c and/or C 16:1 ω 7; 7.9 %) and C 18:1 w 9 c (4.7 %) as the major fatty acids. The polar lipids were found to consist of two aminolipids, a glycolipid, a phospholipid, a phosphoglycolipid, phosphatidylcholine, phosphatidylethanolamine and diphosphatidylglycerol. Strain BUT-13 T showed low DNA–DNA relatedness with R. pecuniae N75 T (45.2 %) and R. rosea 173-96 T (51.2 %). The DNA G+C content was determined to be 67.6 mol%. Based on the phylogenetic analysis, DNA–DNA hybridization and chemotaxonomic analysis, as well as biochemical characteristics, strain BUT-13 T can be clearly distinguished from all currently recognised Roseomonas species and should be classified as a novel species of the genus Roseomonas , for which the name Roseomonas chloroacetimidivorans sp. nov. is proposed. The type strain is BUT-13 T (CCTCC AB 2015299 T = JCM 31050 T ).