Predictive Role Of Galectin-1 And Integrin Alpha 5 Beta 1 In Cisplatin-Based Neoadjuvant Chemotherapy Of Bulky Squamous Cervical Cancer

Although galectin-1 and integrin alpha 5 beta 1 confer chemoresistance to certain types of cancer, whether their expression predicts the response to cisplatin-based neoadjuvant chemotherapy (NACT) in squamous cervical cancer remains unclear. Paired tumor samples (pre- and post-chemotherapy) were obtained from 35 bulky squamous cervical cancer patients treated with cisplatin-based NACT and radical hysterectomy at our hospital between January 2007 and August 2014. The expression of galectin-1 and integrin alpha 5 beta 1 in tumor cells and stromal cells was analyzed by immunohistochemistry. The correlation between galectin-1/integrin alpha 5 beta 1 and apoptosis-associated markers was investigated by using the The Cancer Genome Atlas (TCGA) RNA-sequencing data. Seventeen patients were identified as chemotherapy responders and 18 as non-responders. Galectin-1 and integrin alpha 5 beta 1-positive immunostaining was more frequently observed in stromal cells than its in tumor cells. The expression of galectin-1 and integrin alpha 5 beta 1 in stromal and tumor cells was significantly down-regulated in postchemotherapy cervical cancer tissues. High levels of galectin-1 and integrin alpha 5 beta 1 in stromal were associated with a negative chemotherapy response in squamous cervical cancer patients treated with cisplatin-based NACT. Additionally, the expression of galectin-1 and integrin alpha 5 correlated negatively with caspase 3/caspase 8 by using the TCGA RNA-sequencing data. Galectin-1 and integrin alpha 5 beta 1 expression in stromal may serve as a prediction of the responses to cisplatin-based NACT for patients with bulky squamous cervical cancer. Galectin-1 and integrin alpha 5 beta 1 may be implicated in the development of chemoresistance in cervical cancer via suppressing apoptosis.