Comparison of Immunogenic Efficacy of Mono- and Polyvalent Rabies Vaccines in Dogs

Monovalent and polyvalent vaccines are commonly used rabies cell culture vaccines that can vary in their immunogenic efficacy in dogs. In this study, immunogenic efficacy of two monovalent vaccines (Rabisin and Rabisyva-VP13) and a polyvalent vaccine (Hexadog DHP-LR) was compared. Sixteen adult, healthy, non-vaccinated dogs were randomly assigned to 4 groups (A to D). Dogs in groups A, B, and C were inoculated with Rabisin (1 dose SIC), Hexadog DHP-LR (1 dose S/C), and Rabisyva-VP13 (1 dose S/C), respectively, at day 0 and 21. Dogs in,group D were maintained as negative controls (non-vaccinated). Serum samples were collected on days of vaccination (0 and 21) and every 30 days for up to 300 days. Rabies virus neutralizing antibody (RVNA) titers were determined by Rapid Florescent Focus Inhibition Test (RFFIT). The monovalent vaccines were found to generate higher RVNA titers than the polyvalent vaccine. Vaccine type and post vaccination intervals significantly affected the RVNA titers. Gender of dogs did not affect vaccine efficacy except that gender had some effect on RVNA titers generated by polyvalent vaccinated dogs.