What is the place of oxidative stress test in prediction of clinical pregnancy in ART cycles?

ObjectiveOxidative stress is considered to be the main factor in the development of placental related diseases such as abortus and intrauterine growth retardation. The aim of this study was to evaluate the role of oxidative stress in development of clinical pregnancy in ART cycles.DesignProspective observational study in an University Hospital.Materials and Methods29 male factor infertile couple who were candidates for ICSI were enrolled. A long gonadotropin-releasing hormone agonist protocol was used for ovarian stimulation. The total oxidant status (TOS) and antioxidant status (TAS) of plasma were measured using a novel automated colorimetric method developed by Erel. TAS and TOS levels were measured before oocyte collection-after oocyte collection and also before embryo transfer-after embryo transfer. TAS and TOS levels were compared in clinically pregnant and non-pregnant women.ResultsTable 1TAS levels before-after oocyte collection, before-after embryo transfer in clinically pregnant and non-pregnant womenClinically pregnant (n=9)Clinically non-pregnant (n=20)P valueTAS before oocyte collection1,7000 (1,5550-1,7050)1,5550 (1,5400-1,5975)0.035TAS after oocyte collection1,6600 (1,5800-1,6850)1,5800 (1,5700-1,6200)0.010TAS before embryo transfer1,6533±,072631,5945±,054140.022TAS after embryo transfer1,6878±,059111,6275±,052400.010TAS (μmol H2O2 Eq/L) levels as Mean±Std. Deviation and Median (25%-75%), P-value <0.05 was considered statistically significant. Open table in a new tab ConclusionOxidative stress most probably effects development of clinical pregnancy in ART cycles. TAS levels may predict clinical pregnancy. With taking measures for about increasing TAS during ART cycles may result with increased clinical pregnancies. ObjectiveOxidative stress is considered to be the main factor in the development of placental related diseases such as abortus and intrauterine growth retardation. The aim of this study was to evaluate the role of oxidative stress in development of clinical pregnancy in ART cycles. Oxidative stress is considered to be the main factor in the development of placental related diseases such as abortus and intrauterine growth retardation. The aim of this study was to evaluate the role of oxidative stress in development of clinical pregnancy in ART cycles. DesignProspective observational study in an University Hospital. Prospective observational study in an University Hospital. Materials and Methods29 male factor infertile couple who were candidates for ICSI were enrolled. A long gonadotropin-releasing hormone agonist protocol was used for ovarian stimulation. The total oxidant status (TOS) and antioxidant status (TAS) of plasma were measured using a novel automated colorimetric method developed by Erel. TAS and TOS levels were measured before oocyte collection-after oocyte collection and also before embryo transfer-after embryo transfer. TAS and TOS levels were compared in clinically pregnant and non-pregnant women. 29 male factor infertile couple who were candidates for ICSI were enrolled. A long gonadotropin-releasing hormone agonist protocol was used for ovarian stimulation. The total oxidant status (TOS) and antioxidant status (TAS) of plasma were measured using a novel automated colorimetric method developed by Erel. TAS and TOS levels were measured before oocyte collection-after oocyte collection and also before embryo transfer-after embryo transfer. TAS and TOS levels were compared in clinically pregnant and non-pregnant women. ResultsTable 1TAS levels before-after oocyte collection, before-after embryo transfer in clinically pregnant and non-pregnant womenClinically pregnant (n=9)Clinically non-pregnant (n=20)P valueTAS before oocyte collection1,7000 (1,5550-1,7050)1,5550 (1,5400-1,5975)0.035TAS after oocyte collection1,6600 (1,5800-1,6850)1,5800 (1,5700-1,6200)0.010TAS before embryo transfer1,6533±,072631,5945±,054140.022TAS after embryo transfer1,6878±,059111,6275±,052400.010TAS (μmol H2O2 Eq/L) levels as Mean±Std. Deviation and Median (25%-75%), P-value <0.05 was considered statistically significant. Open table in a new tab TAS (μmol H2O2 Eq/L) levels as Mean±Std. Deviation and Median (25%-75%), P-value <0.05 was considered statistically significant. ConclusionOxidative stress most probably effects development of clinical pregnancy in ART cycles. TAS levels may predict clinical pregnancy. With taking measures for about increasing TAS during ART cycles may result with increased clinical pregnancies. Oxidative stress most probably effects development of clinical pregnancy in ART cycles. TAS levels may predict clinical pregnancy. With taking measures for about increasing TAS during ART cycles may result with increased clinical pregnancies.