Roseomonas Wooponensis Sp Nov., Isolated From Wetland Freshwater

A non-motile, cocobacilli-shaped and pink-pigmented bacterium, designated strain WW53(T), was isolated from wetland freshwater (Woopo wetland, Republic of Korea). Cells were Gram-stain-negative, catalase-and oxidase-positive. The major fatty acids were C-18 : 1 omega 7c/C-18 : 1 omega 6c and C-16 : 0. The predominant quinone and polyamine were ubiquinone 10 (Q-10) and spermidine, respectively. The DNA G + C content was 71 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and an unknown aminolipid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WW53(T) belongs to the family Acetobacteraceae, and is related to the genus Roseomonas. Strain WW53(T) was most closely related to Roseomonas stagni HS-69(T) (95.3 % 16S rRNA gene sequence similarity). Results of a polyphasic taxonomy study suggested that the isolate represents a novel species in the genus Roseomonas, for which the name Roseomonas wooponensis sp. nov. is proposed. The type strain is WW53(T) (= KCTC 32534(T) = JCM 19527(T)).