Characteristic time courses of cortical and medullary sodium signals measured by noninvasive (23) Na-MRI in rat kidney induced by furosemide.

BackgroundTo characterize regional kidney sodium response by MRI following NKCC2 inhibition. MethodsRegional renal sodium signals were monitored noninvasively using Na-23-MRI at 9.4T with a temporal resolution of 1.5 min in anesthetized rats (N=14). A mild NKCC2 inhibition was induced using a slow intravenous furosemide infusion. Time course of sodium signal was modeled as an exponential transient with a single characteristic time constant. ResultsUnder normal physiological conditions, the renal sodium signals in medullary and cortical regions were stable and found to respond differently to furosemide challenge. Furosemide infusion at 1.2 mg/kg/h (N=7) increased sodium signal in the cortex by 406% (P<7 x 10(-5)) whereas decreased in the medulla by 29 +/- 2% (P<3 x 10(-6)) with different temporal kinetics. The characteristic time constants of the change were determined to be: 8 +/- 2 and 70 +/- 10 min for medulla and cortex. Also, the medullary change occurred 9(+/- 3) times faster than cortical independent of furosemide infusion rate up to 35 mg/kg/h. ConclusionThe pharmacological effects in terms of regional kidney sodium signal changes induced by NKCC2 inhibition are region-specific and highly predictable. Using noninvasive sodium MRI, we obtained regional renal sodium kinetics data sets in response to a low dose furosemide infusion in normal rats. J. Magn. Reson. Imaging 2015;41:1622-1628. (c) 2014 Wiley Periodicals, Inc.