Development of an electrochemical sensor based on the catalysis of ferrocene actuated hemin/G-quadruplex enzyme for the detection of potassium ions.
Biosensors & bioelectronics(2014)
摘要
Sensitive and selective sensors need to be explored to detect the physiological potassium level due to its important role in the living organisms. In the present system, a novel electron transfer mediator actuated electrocatalytical biosensor was demonstrated to assay K(+) based on the conformational change of DNA. With the hybridization between the complementary bases and the self-folding of guanine-rich nucleic acid sequence, the horseradish peroxidase-mimicking enzyme (HRP-DNAzyme) was formed and brought to approach the ferrocene (Fc) unit on Au nanoparticles (AuNPs). Thus, in the system, Fc unit acted as the relay, stimulating the electrical contact of HRP-DNAzyme with the electrode to obtain the bioelectrocatalyze reduction signal. Under the Fc actuated catalysis of HRP-DNAzyme and amplification of Au nanoparticles, the obtained biosensor exhibited a sensitive detection for K(+). A satisfying result of a wide linear range and low detection limit were obtained with the novel electrocatalytical biosensor which was then applied in real samples.
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