DNase I treatment of cDNA first strands prevents RT-PCR amplification of contaminating DNA sequences.

BioTechniquesVol. 35, No. 5 BenchmarksOpen AccessDNase I treatment of cDNA first strands prevents RT-PCR amplification of contaminating DNA sequencesAljoscha M. Flohr, Thomas Hackenbeck, Claudia Schlueter, Piere Rogalla & Joern BullerdiekAljoscha M. FlohrCenter for Human Genetics, University of BremenSearch for more papers by this author, Thomas HackenbeckCenter for Human Genetics, University of BremenSearch for more papers by this author, Claudia SchlueterCenter for Human Genetics, University of BremenSearch for more papers by this author, Piere Rogallaalcedo biotech, Bremen, GermanySearch for more papers by this author & Joern Bullerdiek*Address correspondence to Joern Bullerdiek, Center for Human Genetics, University of Bremen , Leobenerstr. ZHG, D-28359 Bremen, Germany. e-mail: E-mail Address: bullerdiek@unibremen.deCenter for Human Genetics, University of BremenSearch for more papers by this authorPublished Online:24 Sep 2018https://doi.org/10.2144/03355bm03AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByQuantitative PCR techniquesQuantitative PCR TechniquesQuantitative PCR TechniquesContaminating insert degradation by preincubation colony PCR: A method for avoiding false positives in transformant screeningAnalytical Biochemistry, Vol. 375, No. 1Gel Purification of Genomic DNA Removes Contaminating Small DNA Fragments Interfering with Polymerase Chain Reaction Analysis of Small Fragment Homologous ReplacementOligonucleotides, Vol. 16, No. 4Urea-nuclease treatment of concentrated retrovirions preserves viral RNA and removes polymerase chain reaction-amplifiable cellular RNA and DNAJournal of Virological Methods, Vol. 137, No. 2The Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes Are Not Expressed in the Rat HeartEndocrinology, Vol. 146, No. 12Quality assessment of maize assembled genomic islands (MAGIs) and large-scale experimental verification of predicted genes15 August 2005 | Proceedings of the National Academy of Sciences, Vol. 102, No. 34Immobilization of deoxyribonuclease via epoxy groups of methacrylate monolithsJournal of Chromatography A, Vol. 1065, No. 1Quantitative PCR TechniquesMethod based on electrophoresis and gel extraction for obtaining genomic DNA-free cDNA without DNase treatmentLaura Jaakola, Anna Maria Pirttilä, Jaana Vuosku & Anja Hohtola6 June 2018 | BioTechniques, Vol. 37, No. 5 Vol. 35, No. 5 Follow us on social media for the latest updates Metrics Downloaded 287 times History Published online 24 September 2018 Published in print November 2003 Information© 2018 Author(s)PDF download