Feasibility Of Direct Mapping Of Cerebral Fluorodeoxy-D-Glucose Metabolism In Situ At Subcellular Resolution Using Soft X-Ray Fluorescence
JOURNAL OF NEUROSCIENCE RESEARCH(2013)
摘要
Glucose metabolism is difficult to image with cellular resolution in mammalian brain tissue, particularly with 18fluorodeoxy-D-glucose (FDG) positron emission tomography (PET). To this end, we explored the potential of synchrotron-based low-energy X-ray fluorescence (LEXRF) to image the stable isotope of fluorine (F) in phosphorylated FDG (DG-6P) at 1 m2 spatial resolution in 3-m-thick brain slices. The excitation-dependent fluorescence F signal at 676 eV varied linearly with FDG concentration between 0.5 and 10 mM, whereas the endogenous background F signal was undetectable in brain. To validate LEXRF mapping of fluorine, FDG was administered in vitro and in vivo, and the fluorine LEXRF signal from intracellular trapped FDG-6P over selected brain areas rich in radial glia was spectrally quantitated at 1 m2 resolution. The subsequent generation of spatial LEXRF maps of F reproduced the expected localization and gradients of glucose metabolism in retinal Muller glia. In addition, FDG uptake was localized to periventricular hypothalamic tanycytes, whose morphological features were imaged simultaneously by X-ray absorption. We conclude that the high specificity of photon emission from F and its spatial mapping at 1 m resolution demonstrates the ability to identify glucose uptake at subcellular resolution and holds remarkable potential for imaging glucose metabolism in biological tissue. (c) 2012 Wiley Periodicals, Inc.
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关键词
fluorodeoxy-D-glucose, low-energy X-ray fluorescence spectromicroscopy, periventricular hypothalamus, radial glia, retina, synchrotron
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