Cell Surface Expression Of Hfe Protein In Epithelial Cells, Macrophages, And Monocytes

Background and Objectives. Most patients with hereditary hemochromatosis are homozygous for a Cys282 --> Tyr mutation In the HFE gene. This mutation has been shown to impair the association of the HFE gene product with beta(2)-microglobulin and to prevent its cell surface presentation in transfected COS-7 and 293 cells. This study was performed to line the expression of HFE protein in epithelial macrophages, and circulating leukocytes obtained from normal subjects and patients with hereditary hemochromatosis,Design and Methods. Antisera against two different peptides of the HFE protein were used to immunostain tissue sections and isolate granulocytes, lymphocytes and monocytes.Results. Immunocytochemical staining showed that HFE protein Is expressed in gastric epithelial cells, tissue macrophages, and circulating monocytes and granulocytes, The cell surface associated signal, which was seen in normal gastric epithelial cells, monocytes and macrophages, was also present in C282Y mutant cells from patients with hereditary hemochromatosis, although at apparently reduced amounts in these cells.Interpretation and Conclusions. From these studies, It is clear that the C282Y mutation reduces but does not completely prevent presentation of the HFE protein on the cell surface of human monocytes, tissue macrophages, and gastric epithelial cells. (C) 2000, Ferrata Storti Foundation.