Nitric Oxide-Induced Activation Of The Amp-Activated Protein Kinase Alpha 2 Subunit Attenuates I Kappa B Kinase Activity And Inflammatory Responses In Endothelial Cells

Background: In endothelial cells, activation of the AMP-activated protein kinase (AMPK) has been linked with anti-inflammatory actions but the events downstream of kinase activation are not well understood. Here, we addressed the effects of AMPK activation/deletion on the activation of NF kappa B and determined whether the AMPK could contribute to the anti-inflammatory actions of nitric oxide (NO).Methodology/Principal Findings: Overexpression of a dominant negative AMPK alpha 2 mutant in tumor necrosis factor-alpha-stimulated human endothelial cells resulted in increased NF kappa B activity, E-selectin expression and monocyte adhesion. In endothelial cells from AMPK alpha 2(-/-) mice the interleukin (IL)-1 beta induced expression of E-selectin was significantly increased. DETA-NO activated the AMPK and attenuated NF kappa B activation/E-selectin expression, effects not observed in human endothelial cells in the presence of the dominant negative AMPK, or in endothelial cells from AMPK alpha 2(-/-) mice. Mechanistically, overexpression of constitutively active AMPK decreased the phosphorylation of I kappa B and p65, indicating a link between AMPK and the I kappa B kinase (IKK). Indeed, IKK (more specifically residues Ser177 and Ser181) was found to be a direct substrate of AMPK alpha 2 in vitro. The hyper-phosphorylation of the IKK, which is known to result in its inhibition, was also apparent in endothelial cells from AMPK alpha 2(+/+) versus AMPK alpha 2(-/-) mice.Conclusions: These results demonstrate that the IKK is a direct substrate of AMPK alpha 2 and that its phosphorylation on Ser177 and Ser181 results in the inhibition of the kinase and decreased NF kappa B activation. Moreover, as NO potently activates AMPK in endothelial cells, a portion of the anti-inflammatory effects of NO are mediated by AMPK.