Activation Of The Regulator Of G Protein Signaling 14-G Alpha I1-Gdp Signaling Complex Is Regulated By Resistance To Inhibitors Of Cholinesterase-8a

RGS14 is a brain scaffolding protein that integrates G protein and MAP kinase signaling pathways. Like other RGS proteins, RGS14 is a GTPase activating protein (GAP) that terminates G alpha i/o signaling. Unlike other RGS proteins, RGS14 also contains a G protein regulatory (also known as GoLoco) domain that binds G alpha i1/3-GDP in cells and in vitro. Here we report that Ric-8A, a nonreceptor guanine nucleotide exchange factor (GEF), functionally interacts with the RGS14-G alpha i1-GDP signaling complex to regulate its activation state. RGS14 and Ric-8A are recruited from the cytosol to the plasma membrane in the presence of coexpressed G alpha i1 in cells, suggesting formation of a functional protein complex with G alpha i1. Consistent with this idea, Ric-8A stimulates dissociation of the RGS14-G alpha i1-GDP complex in cells and in vitro using purified proteins. Purified Ric-8A stimulates dissociation of the RGS14-G alpha i1-GDP complex to form a stable Ric-8A-G alpha i complex in the absence of GTP. In the presence of an activating nucleotide, Ric-8A interacts with the RGS14-G alpha i1-GDP complex to stimulate both the steady-state GTPase activity of G alpha i1 and binding of GTP to G alpha i1. However, sufficiently high concentrations of RGS14 competitively reverse these stimulatory effects of Ric-8A on G alpha i1 nucleotide binding and GTPase activity. This observation correlates with findings that show RGS14 and Ric-8A share an overlapping binding region within the last 11 amino acids of G alpha i1. As further evidence that these proteins are functionally linked, native RGS14 and Ric-8A coexist within the same hippocampal neurons. These findings demonstrate that RGS14 is a newly appreciated integrator of unconventional Ric-8A and G alpha i1 signaling.