Titration Of A Vaccine Stock Preparation Of Human Immunodeficiency Virus Type 1(Sf2) In Cultured Lymphocytes And In Chimpanzees

A large stock preparation of the HIV-1(SF2) isolate has been derived after serial passage in human peripheral blood mononuclear cells (PBMCs). This viral stock has a titer of 10(4.9) TCID50 in human PBMCs and 10(4.2) TCID50 in chimpanzee PBMCs, Bg inoculation into animals the 50% chimpanzee infectious dose titer was found to be about 10(2.3). Virus isolation from animals was achieved on most occasions within 1-4 weeks after inoculation and then became transient, Viral RNA and DNA PCR analyses confirmed the virus infection of the chimpanzees, Anti-HIV antibody levels in the inoculated animals ranged from 1:400 to 1:6400 as measured by ELISA, About 680 vials of this stock preparation, frozen at -190 degrees C, are available for future studies of vaccines and antiviral therapies.