ISSR analysis on genetic diversity of Taraxaci Herba

Objective: To analyze the genetic diversity of Taraxaci Herba. Methods: Genetic diversity of 21 populations of Taraxaci Herba was analyzed by ISSR. Nei's genetic diversity index (H) and Shannon's information index (I) were calculated by Popgen 32. Cluster and principal coordinate analyses were completed by NTSYS 2.10e software. Results: A total of 166 bands were amplified by 16 ISSR primers, among which 152 were polymorphic bands (PPB). The percentage of PPB was 90.4%. The average H and I were 0.2865 and 0.4370, respectively. The genetic distance and genetic similarity were 0.1562-0.590 2 and 0.5542-0.8554, respectively. UPGMA cluster analysis showed that the geographical distribution was mutually related to the cluster results. It also showed that some of Taraxaci Herba from the same region were in the same group which presented the rule of the geographical distribution. Conclusion: ISSR analysis reveals that Taraxaci Herba has high genetic diversity. The ISSR analysis could be used to study of the genetic diversity of Taraxaci Herba at molecular level.