The changes of CD4~+CD25~+ regulatory T cells in peripheral blood cells and the allergic state of mites in bronchial asthma

This study is assigned to investigate the changes of CD4+CD25+ regulatory T cells in peripheral blood cells and the allergic state of mites of bronchial asthma.We used the peripheral blood from bronchial asthma and healthy subjects as samples to measure the CD4+CD25+ Tr levels with flow cytometry(FCM).The allergic state of mites of bronchial asthma was tested by skin prick test.We found that the CD4+CD25+ Tr levels(4.81% ± 2.76%) in acute asthmatic group were lower than that in the control group(8.66% ± 1.61%)(P 0.01),while the CD4+CD25+ Tr levels(2.73% ± 2.03%) in the acute asthmatic group with(+++) positive skin prick test of mites were lower than those with(+…++) positive skin prick test of mites(6.45% ± 2.08%)(P 0.01).The results show that the levels of CD4+CD25+ Tr remarkable decrease in acute bronchial asthma,and even more decrease in patients with allergic physique.Thus we presumed there is remarkably negative correlation between the levels of CD4+CD25+ Tr and the allergic state in acute bronchial asthma,indicating that CD4+CD25+ Tr is related to the allergic state of bronchial asthma.