Study of chromium-containing proteins in subcellular fractions of rat liver by enriched stable isotopic tracer technique and gel filtration chromatography
Analytical and Bioanalytical Chemistry(2003)
摘要
Ten male Wistar rats were intravenously injected with a single approximately physiological dose of enriched stable isotopic Cr-50 tracer solution (200 ng 50 Cr 3+ /100 g body wt). The fundamental distribution patterns of the chromium-containing proteins in the nucleic, mitochondrial, lysosomal, microsomal, and cytosolic subcellular fractions of the rat liver were investigated by means of Sephadex G-100 gel chromatography combined with neutron activation analysis via 50 Cr (n, γ) 51 Cr reaction. In total, nine kinds of Cr-containing proteins were found in the five subcellular fractions, whose relative molecular masses were 96.6±6.2, 68.2±1.4, 57.9±4.7, 36.6±1.2, 24.2±1.8, 14.0±1.5, 8.8±0.6, 6.9±0.4, and 4.2±0.4 kDa. Approximately 64.5% of Cr proteins accumulated in the cytosolic fraction. The second enriched part was the nucleic fraction; about 12.2% Cr proteins were stored in this section. The 4.2-kDa molecular mass might contain the so-called low molecular weight chromium-containing substance; however, in this research, it was only observed in the mitochondria, lysosome, and microsome. In the mitochondrial fraction, most of the Cr proteins were present as relatively low molecular weight substances: about 56% of chromium-containing proteins had molecular masses ≤6.9 kDa. Nevertheless, more than 69% of Cr-containing proteins were observed with molecular masses ≥57.9 kDa in the liver cytosolic fraction.
更多查看译文
关键词
Cr-containing protein,Speciation,Enriched stable isotopic tracer technique,Subcellular,Rat
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要