Dysbiosis Characterized by Reduced Abundance of Roseburia is Associated With Increased Severity of Colitis in IL-10−/− Mice

G A A b st ra ct s estimated by real-time PCR assay using oligonucleotide primers specific to immediate early gene. More than 10 copies/μg DNA of PCR amplicons were defined as positive. We compared the positive ratio of those colonoscopic findings of UC patients between positive and negative for CMV-DNA. Additionally, we examined the correlation between the number of CMVDNA amplicons [negative, low-copy group (10-100 copies/μg DNA), high-copy group (≧100 copies/μg DNA)], and colonoscopic features and their medications. Results: Positive ratio of ulcerative lesions was higher in CMV-DNA-positive group than in CMV-DNA-negative group [22/34 (64.7%) vs. 10/27 (37.0%), p<0.05]. Further analysis in limited to UC patients who had no ulcerative lesions revealed that the positive ratio of edema or redness in CMVDNA-positive groupwere significantly higher than in CMV-DNA-negative UC group [redness, 8/12 (66.7%) vs. 4/17 (23.5%); edema, 7/12 (58.3%) vs. 2/17 (11.8%)]. Moreover, the positive ratio of edema or redness is higher even in low-copy group of CMV-DNA-positive UC patients (Table). Ratio of UC patients treated with the combination of steroids and immunomodulators is significantly higher in the high-copy group than in other groups [negative, 3/27 (11.1%); low-copy group, 1/10 (10.0%); high-copy group, 11/24 (45.8%)]. Conclusions: In addition to ulcerative lesions, reddish and edematous colonic mucosa might be characteristic of early colonoscopic features in UC patients concomitant with CMV reactivation. CMV reactivation can be easily occurred in patients with UC refractory to the combined therapies of steroids and immunomodulators.