HPLC Determination of a Vincristine, Doxorubicin, and Ondansetron Mixture in 0.9% Sodium Chloride Injection

A high performance liquid chromatography procedure has been developed for the assay of a vincristine, doxorubicin, and ondansetron mixture in 0.9% sodium chloride injection. The separation and quantitation are achieved on a phenyl column at ambient temperature using a mobile phase of 50:50 v/v 0.02 M phosphate buffer, pH 5.4-acetonitrile at a flow rate of 1.0 mL/min with detection of all three analytes at 233 nm. The separation is achieved within 15 min with sensitivity in the ng/mL range for each analyte. The method showed linearity for vincristine, doxorubicin, and ondansetron in the 0.36 - 3.6, 10.0 - 100, and 11.95 - 119.7 mu g/mL ranges, respectively. Accuracy and precision were in the 1 - 3% and 0.2 - 3.3% ranges, respectively, for all three compounds. The limits of detection for vincristine, doxorubicin, and ondansetron were 90, 200 and 200 ng/mL, respectively, based on a signal to noise ratio of 3 and a 20 mu L injection.