The research on the comparability of the conventional biochemical test results using different test systems

Objective To discuss the conventional biochemical detecting results between three different systems,and provide evidence for clinical laboratory recognition.Methods Referring to the clinical laboratory standardization commission(NCCLS) EP9-A document,and taking HITACHI 7600 automatic biochemistry analyzer,roche diagnostics,cfas calibration and control product Randox British system as the target systems,we compared the results of fresh specimens of patients in different experimental detection systems on biochemical test of 14 routine with the result of target detection systems.We also measured the relative bias between the experimental comparison testing system(Y) and target detection system(X),and the total error analysis(TEa) of 1/2 according to the revised regulations of clinical inspection department of America(CLIA88) was taken as the standard to judge the comparability of the detection results.Results Detected by the experiment test system one(Y1),the difference of results between serum samples TG and TC was significant(P0.05), 1/2 TEa,and the difference of results among BUN 2 mmol/L level,Cr 50 μmol/L level,Tb and DB was statistically significant(P0.05), 1/2 TEa.Plasma samples of ALT difference was statistically significant as well(P0.05), 1/2 TEa,and the rest results were in accordance with that in blood serum.The difference of results among TB,BC and LDH in experiment test system two(Y2) was significant(P0.05), 1/2 TEa,which had no connection with the type of the samples.Conclusion The results of conventional biochemical detection by different systems on some items have various degrees of deviation.Using different detection systems to detect the same item,we should undertake comparative and clinical acceptability analysis on different methods,and take ameliorative actions to those do not have comparability for the test results.