Hypertonic resuscitation of hemorrhagic shock prevents alveolar macrophage activation by preventing systemic oxidative stress due to gut ischemia/reperfusion

Background. The gut is a target organ of shock/resuscitation (SIR); however, it also contributes to distant inflammation through the generation of oxidants. SIR with antioxidants such as N-acetylcysteine (NAC) prevents lipopolysaccharide (LPS)-induced cytokine production and NF-kappaB activation in rat alveolar macrophages. Therefore, we hypothesized that hypertonic saline (HTS) might exerts its Protective effect by preventing g-ut ischemia/reperfusion injury, thus decreasing oxidative stress and distant priming in alveolar macrophages. Methods. A two-hit rat model of shock resuscitation was used. Plasma levels of 8-iso-prostaglandin, a marker of lipid peroxidation, was quantified by eicosanoid immunoassay with acetylcholinesterase kit. Gut histology with hematoxylin and eosin staining was performed I to 6 hours after resuscitation. Alternatively, alveolar macrophages from bronchoalveolar lavage (BAL) at end resuscitation were incubated in vitro with LPS (0.01 mug/mL), and NF-kappaB translocation was observed by immunofluorescent staining with anti-P65 antibody. Results. HTS resuscitation prevented leukosequestration in the alveolar space, and it abrogated the Progressive rise in blood 8-iso-prostoglandin production observed with Ringer's lactate (RL) resuscitation. Inhibition of oxidant stress with NAC corresponded with the ability of HTS to prevent SIR-induced edema, villus flattening, and mucosal sloughing in the mid-ileum. LPS-induced NF-kappaB translocation in alveolar macrophages after RL was 42 % compared to 20 % after HTS. Similar attenuation was observed with NAC resuscitation (16 %). Conclusions. HTS resuscitation prevents systemic oxidative stress by reducing gut ischemial reperfusion injury and consequently attenuates distant alveolar macrophage priming, thereby reducing LPS-induced NF-kappaB nuclear translocation in alveolar macrophages and organ injury. This represents a novel mechanism whereby HTS exerts its immunomodulatory effects.