Bradykinin stimulates the intracellular calcium activity in human mesothelial cells.
NEPHROLOGY DIALYSIS TRANSPLANTATION(1998)
摘要
Aim. The aim of this study was to investigate the influence of bradykinin on the intracellular calcium activity ([Ca2+](i)) in human mesothelial cells in culture. Results. Bradykinin (1-1000 nmol/l) caused a concentration-dependent and reversible increase of [Ca2+](i) in mesothelial cells (n=94); 10 nmol/l bradykinin increased [Ca2+](i) from 23 +/- 9 to 670 +/- 170 nmol/l (n = 36). The pattern of the bradykinin-induced [Ca2+](i) increase was biphasic with a transient [Ca2+](i) peak, which was followed by a sustained [Ca2+](i) plateau. The bradykinin-mediated [Ca2+](i) plateau, but not the peak, was inhibited in a solution with an extracellular reduced Ca2+ concentration (from 1000 to 1 mu mol/l, n=11). Flufenamate (greater than or equal to 10 mu mol/l), an inhibitor of non-selective ion channels abolished the bradykinin-mediated increase of [Ca2+](i), whereas the L-type Ca2+ channel blocker nicardipine (10 mu mol/l) had no effect (n=3-5). The [Ca2+](i) response to bradykinin was inhibited by the BK, antagonist Hoe 140 (IC50 +/- k7 nmol/l, n = 30). Conclusions. The data indicate that bradykinin stimulates [Ca2+](i) in mesothelial cells by a release of Ca2+ from intracellular stores and an influx from Ca2+ through non-selective channels via a BK2 receptor.
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关键词
bradykinin,mesothelial cells,intracellular calcium activity
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