Lysophosphatidic acid induces protein tyrosine phosphorylation in the absence of phospholipase C activation in human platelets

Lysophosphatidic acid is a biologically active phospholipid able to induce cell proliferation and platelet aggregation, In this study we investigated the biochemical mechanisms of platelet activation by lysophosphatidic acid, We found that lysophosphatidic acid stimulated the binding of the photoreactive GTP-analog 4-azidoanilido-[alpha(32)P]GTP to a 40-kDa protein on platelet membranes, Moreover, lysophosphatidic acid induced the rapid decrease of the intracellular concentration of cAMP in intact platelets, indicating that this lipid activates platelets by binding to a membrane receptor coupled to the inhibitory GTP-binding protein Gi, In agreement with a receptor-mediated action, we found that platelet activation by lysophosphatidic acid underwent homologous desensitization. In the absence of extracellular CaCl2, lysophosphatidic acid did not induce platelet aggregation, and did not stimulate phospholipase C, However, under the same conditions, lysophosphatidic acid produced the rapid tyrosine phosphorylation of several platelet proteins, This effect was not mediated by the formation of thromboxane A(2), Our results demonstrate that, in lysophosphatidic acid-stimulated platelets, activation of protein-tyrosine kinases occurs in the absence of phospholipase C activation and platelet aggregation, and may be directly related to the activation of the G-protein-coupled lysophosphatidic acid-receptor.