cDNA Cloning,Sequence Analysis and Tissue Expression of Duck CYP7_α1 Gene

This study was carried out to obtain and analyze sequence of duck CYP7α1 gene,and to reveal the expression of CYP7α1 mRNA in different tissues of duck.Based on other species mRNA sequences,the cDNA of CYP7α1 gene in duck was amplified successfully by using RACE-PCR.The structure and the function of the duck CYP7α1 gene were analyzed by bioinformatics methods.The mRNA abundance of duck CYP7α1 gene was tested in 10 tissues.The result showed that the cDNA of duck CYP7α1 gene was 2 351 nucleotides in length with an open reading frame(ORF) 1 539 bp(114-1 652 bp) in length and encoded 512 amino acids.The amino acid homology of duck CYP7α1 were 97.5%,92.8%,67.3%,66.5%,65.7%,65.5%,65.5% and 65.1% comparing to that of the goose,chicken,human,pig,rabbit,rat,mouse and cattle,respectively.The CYP7α1 amino acid sequence of duck contained a P450 domain.The mRNA expression level of duck CYP7α1 gene was high in liver and was not detected in other 9 tissues,the mRNA expression of duck CYP7α1 gene was tissue specific.