Isolation of alveolar epithelial cells from lung tissue obtained at autopsy
In vitro cellular & developmental biology : journal of the Tissue Culture Association(1989)
摘要
Summary Lung alveolar epithelial cells have been studied in a variety of laboratory animal models, and studies of human alveolar epithelial
cells are important for comparison to information obtained from animal studies. Autopsy material is a source of human cells
for study. Studies of human autopsy material revealed variables that negatively affected the yield of viable cells. For specimens
from adults, these included death greater than 12 h before cell isolation, obvious severe lung fibrosis, longstanding metabolic
disorders, and lung congestion indicated by weight of the right middle lobe greater than 150 g. Samples from children yielded
significant numbers of viable cells up to 18 h after death. For 17 specimens that conformed to the above criteria, approximately
8.5×106 alveolar cells were obtained per gram of tissue (tissue weights ranged from 30 to 108 g) using a procedure involving instillation
of proteases into the airways. The cells could be further fractionated, and 10 to 15% of the mixed cells obtained were type
II pneumocytes. Analysis of NADPH cytochrome-c-reductase distribution in subcellar fractions provided evidence that the cells
obtained were intact. Phospholipid enzyme activities and synthetic activity were within the ranges previously found in laboratory
studies of freshly obtained animal lungs. These results suggest that significant numbers of viable and functional human lung
cells, including type II pneumocytes, can be obtained from autopsy material.
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关键词
human,pneumocytes,autopsy
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