[Comparative study of three primary culture methods of human epithelial cells of nasal polyps in vitro].

OBJECTIVE:To compare three primary culture methods of human epithelial cells of nasal polyps in vitro, and explore a suitable method to be used in further study. METHOD:Shape and appearance of human epithelial cells of nasal polyps were observed, and success ratio and growth curve of primarily cultural were achieved by enzymatic dissociation, isolated cell ABC culture and tissue piece culture method respectively. RESULT:By comparing achievement ratios, the isolated cell ABC culture method (87.5%) was shown to be superior to enzymatic dissociation (78.13%) and tissue piece culture methods (83.33%), but no statistical significance was found (P>0.05). The growth curves of two isolated cell culture methods were higher than that of tissue piece culture. CONCLUSION:The isolated cell ABC culture model is more suitable for primary culture due to its faster proliferation, less promiscuity, and more stable and reliable cell supply for nasal polyps research.