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    • 小麦DH高效生产技术体系在云南的研究与应用

    YANG Zhonghui,YANG Mujun,LI Shaoxiang,DING Mingliang,LIU Kun,GU Jian,LI Hongsheng

    Journal of Triticeae Crops（2023）

    云南省农业科学院粮食作物研究所

    Cited 0|Views13
    Abstract
    双单倍体(DH)技术可使杂合育种材料在一个世代纯合稳定,被广泛用于作物遗传育种.小麦×玉米杂交是产生小麦DH的主要途径之一,但小麦和玉米一般在不同季节种植,制约了该技术的广泛应用.春性和经春化处理的半冬性、冬性小麦材料在云南昆明 自然条件下一年四季均可播种、收获,为每年4月至12月进行小麦×玉米杂交提供了便利条件.本团队前期建立了平均得胚率为25％(15％～70％)、成苗率为62％(50％～80％)以及加倍率为62％(50％～90％)的小麦DH批量生产技术规程.并于2015年以来,利用国内外共1 900余份不同遗传背景的春性、半冬性和冬性小麦材料进行验证,共获得10.5×104个小麦DH株系;构建了 64个DH遗传群体;育成了云麦110、云麦112等小麦新品种;创制了 24个优良小麦温光敏核不育系及一批抗病优良品系,初步实现了该DH技术在小麦育种中的应用.后续还需继续完善规模化DH生产技术规程,提高得胚率、成苗率、加倍率等关键技术指标和DH生产效率,降低成本,促进该技术在小麦遗传育种中更广泛地应用.
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    Key words
    Wheat× maize,Wide hybridization,Double haploid,Haploid breeding,Yunnan
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